Friday , 21 June 2024

Diagnostic Services

SEAFDEC/AQD offers fish disease diagnostic services such as direct microscopy, PCR diagnosis, bacteriology, mycology, histopathology, and aflatoxin determination.

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Below are the diagnostic services fees:

Bacteriology

Bacterial count (for water, crustaceans, fish fingerlings and juveniles, soil)

Test Fee (per sample)
Aerobic plate count, Luminous bacterial count, and Presumptive Vibrio count 1,2 PHP 300
Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio alginolyticus 1,2 PHP 150
Test Fee (per sample)
Aflatoxin detection (feeds, grain, oil, animal tissue) 3 PHP 500
Parasite detection/identification PHP 150
Bacterial identification (conventional method) PHP 750
Shrimp larval monitoring PHP 250

Histopathology

Service Fee (per slide)
Typical section 4,5 PHP 300
Serial section 4,5 PHP 300

PCR-based pathogen detection

Test Fee (per sample)
Shrimp pathogens  
White spot syndrome virus (WSSV) 6  PHP 900
Infectious hypodermal and hematopoetic necrosis virus (IHHNV) 6  PHP 900
Taura syndrome virus (TSV) 6 PHP 1,000
Yellow head virus (YHV) 6 PHP 1,000
Gill associated virus (GAV) 6 PHP 1,000
Infectious myonecrosis virus (IMNV) 6 PHP 1,000
Monodon baculovirus (MBV) 6 PHP 900
Hepatopancreatic parvo virus (HPV) 6 PHP 900

White tail disease

  • Extra small virus like particles (XSV) 12
  • Macrobrachium rosenbergii nodavirus (MrNV) 13
PHP 900

Shrimp microsporidian parasite

  • Enterocytozoon hepatopenaei (EHP) 6
PHP 900
Quantification of WSSV and IHHNV in soil or tissue 7,8 PHP 3,000
Acute hepatopancreatic necrosis disease (AHPND) 9 PHP 900
Fish pathogens  
Koi herpes virus (KHV) 6 PHP 900
Spring viraemia of carp virus (SVCV) 6 PHP 1,000
Viral nervous necrosis (VNN) 6 PHP 1,000
Iridovirus (red seabream iridovirus/RSIV) 10 PHP 900
Tilapia lake virus (TiLV) 11 PHP 900

To request a fish disease diagnostic from SEAFDEC/AQD:

  1. Contact the Fish Health Section Diagnostic Services for the availability of schedule by sending an email to fishhealth@seafdec.org.ph or call telephone no. (033) 511-9170 loc 1177 / mobile no. (0953)1004474.
  2. Fill up the REQUEST FORM and hand-carry samples to SEAFDEC/AQD or send by registered, door-to-door courier service to:

The Diagnostic Services
Fish Health Section
SEAFDEC/AQD
Tigbauan, 5021 Iloilo
Philippines

  1. Upon receipt of the Statement of Account from the Fish Health Section, you can pay through:
      • Bank transfer: Deposit the computed amount to SEAFDEC/AQD savings account # 023-00-001593-8 at Bank of Commerce-Iznart Branch Iloilo City. Email the bank confirmation or scan of the deposit slip to fishhealth@seafdec.org.ph. Alternatively, fax the deposit slip to (033) 3307011 c/o Fish Health Section.
      • Cash payment: Cash payment is made to the Cashier of SEAFDEC/AQD in Tigbauan, Iloilo by presenting a copy of the Statement of Account. Present the official receipt to the Fish Health Section.
      • Check payment: Checks should be made payable to the Southeast Asian Fisheries Development Center Aquaculture Department and mail to the Fish Health Section together with a copy of the Statement of Account.
  1. The Fish Health Section will release the result, if ready, upon receipt of the proof of payment.

Ways of sending samples:

  1. Live samples

Appropriate for examinations such as:

      • Microscopy
      • Bacteriology
      • Mycology
      • Virology (PCR and tissue culture)
      • Histopathology
  1. Iced samples

Appropriate for examinations such as:

      • Microscopy
      • Bacteriology
      • Mycology
      • Virology
  1. Fixed samples

Appropriate for examinations such as:

      • Virology (PCR)
      • Histopathology

Method of sampling for:

Fish Health Laboratory Services Brochure (2023)
 Download PDF Format
— File Size: 2.66 MB

Watch related video!
Know more about the diagnostic services of SEAFDEC/AQD and how to send samples by watching this video: SEAFDEC/AQD Fish Health Diagnostic Services.

References

1 Standard Methods for the Examination of Water and Waste Waters. 2017. American Public Health Association. 23rd ed.
2 Bacteriological Analytical Manual. US FDA. (online) fda.food/gov/laboratory
3 Aflatoxin rapid test kit (Elabscience, 2018)
4 Humason, G. 1972. Animal Tissue Techniques. 3rd ed. WH Freeman and Company, USA p. 34-47.
5 Bell, T. A. and Lightner, D.V. 1998. A Handbook of normal penaeid shrimp histology. The World Aquaculture Society. Allen Press Inc., Lawrence, Kansas
6 IQ2000TM Detection and Prevention System, GeneReach Biotechnology Corp., Taiwan
7 Tang, KFJ and Lightner, DV. 2001. Detection and quantification of infectious hypodermal and hematopoietic necrosis virus in penaeid shrimp by real-time PCR. Diseases of Aquatic Organisms, 44(2):79-85.
8 Durand, SV and Lightner, DV. 2002. Quantitative real time PCR for the measurement of white spot syndrome virus in shrimp. Journal of Fish Diseases, 25: 381–389.
9 Dangtip S. et al, (2015). AP4 method for two-tube nested PCR detection of AHPND isolates of
Vibrio parahaemolyticus. Aquaculture Reports, 2:158-162.
10 Kurita J, Nakajima K, Hirono I and Aoki T. 1998. Polymerase chain reaction (PCR) amplification of DNA of red sea bream iridovirus (RSIV) Fish Pathol. 33(1):17-23.
11 Dong HT, Siriroob S, Meemetta W, Santimanawong W, Gangnonngiw W, Pirarat N, Khunrae P, Rattanarojpong T, Vanichviriyakit R and Senapin S. 2017. Emergence of tilapia lake virus in Thailand and an alternative semi-nested RT-PCR for detection. Aquaculture 476: 111-118.
12 Sri Widada J, Richard V, Shi Z, Qian D, Bonami JR. Dot-blot hybridization and RT-PCR detection of extra small virus (XSV) associated with white tail disease of prawn Macrobrachium rosenbergii. Dis Aquat Organ. 2004 Jan 28;58(1):83-7
13 Hameed ASS, Yoganandhan K, Widada JS and Bonami JR. 2004. Studies on the occurrence of Macrobrachium rosenbergii nodavirus and extra small virus-like particles associated with white tail disease of M. rosenbergii in India by RT-PCR detection. Aquaculture 238(1–4): pp127-133.

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