SEAFDEC/AQD Southeast Asian Fisheries Development Center | Aquaculture Department
SEAFDEC/AQD offers fish disease diagnostic services such as direct microscopy, PCR diagnosis, bacteriology, mycology, histopathology, and aflatoxin determination.
Below are the diagnostic services fees:
Bacteriology
Bacterial count (for water, crustaceans, fish fingerlings and juveniles, soil)
| Test | Fee (per sample) |
| Aerobic plate count, Luminous bacterial count, and Presumptive Vibrio count 1,2 | PHP 330 |
| Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio alginolyticus detection 1,2 | PHP 200 |
| Bacterial identification | |
| Analytical Profile Index (API) | PHP 1,600 |
| Conventional | PHP 1,700 |
| Microbial sensitivity | PHP 350 |
Fungi
| Test | Fee (per sample) |
| Aflatoxin detection (feeds, grain, oil, animal tissue) 3 | PHP 600 |
Histopathology
| Service | Fee (per slide) |
| Typical section 4,5 | PHP 400 |
| Serial section 4,5 | PHP 400 |
Parasitology
| Test | Fee (per sample) |
| Parasite detection | PHP 150 |
Microscopy
| Test | Fee (per sample) |
| Larval/Fry quality monitoring | PHP 250 |
Media only
| Fee (per plate) | |
| Nutrient Agar (NA) | PHP 30 |
| Thiosulfate Citrate Bile Salt Sucrose Agar (TCBS) | PHP 40 |
| Vibrio Chromogenic Agar (VCA) | PHP 50 |
| Mueller Hinton Agar (MHA) | PHP 30 |
| Pseudomonas–Aeromonas Selective Agar (GSP) | PHP 50 |
PCR-based pathogen detection
| Test | Fee (per sample) |
| Shrimp pathogens | |
| White spot syndrome virus (WSSV) 6 | PHP 900 |
| Infectious hypodermal and hematopoetic necrosis virus (IHHNV) 6 | PHP 900 |
| Taura syndrome virus (TSV) 6 | PHP 1,000 |
| Yellow head virus (YHV) 6 | PHP 1,000 |
| Gill associated virus (GAV) 6 | PHP 1,000 |
| Infectious myonecrosis virus (IMNV) 6 | PHP 1,000 |
| Monodon baculovirus (MBV) 6 | PHP 900 |
| Hepatopancreatic parvo virus (HPV) 6 | PHP 900 |
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White tail disease
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PHP 900 |
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Shrimp microsporidian parasite
|
PHP 900 |
| Acute hepatopancreatic necrosis disease (AHPND) 9 | PHP 900 |
| Fish pathogens | |
| Koi herpes virus (KHV) 6 | PHP 900 |
| Viral nervous necrosis (VNN) 6 | PHP 1,000 |
| Iridovirus (red seabream iridovirus/RSIV) 10 | PHP 900 |
| Tilapia lake virus (TiLV) 11 | PHP 900 |
Quantitative PCR (q-PCR)
| Sample | Fee (per sample) |
| Tissue sample | PHP 3,000 |
| Soil sample | PHP 3,000 |
Nucleic acid extraction
| Test | Fee (per sample) |
| Deoxyribonucleic (DNA) extraction | PHP 300 |
| Ribonucleic (RNA) extraction | PHP 400 |
To request a fish disease diagnostic from SEAFDEC/AQD:
- Contact the Fish Health Section Diagnostic Services for the availability of schedule by sending an email to fishhealth@seafdec.org.ph or call telephone no. (033) 511-9170 loc 1177 / mobile no. (0953)1004474.
- Fill up the REQUEST FORM and hand-carry samples to SEAFDEC/AQD or send by registered, door-to-door courier service to:
The Diagnostic Services
Fish Health Section
SEAFDEC/AQD
Tigbauan, 5021 Iloilo
Philippines
- Upon receipt of the Statement of Account from the Fish Health Section, you can pay through:
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- Bank transfer: Deposit the computed amount to SEAFDEC/AQD savings account # 023-00-001593-8 at Bank of Commerce-Iznart Branch Iloilo City. Email the bank confirmation or scan of the deposit slip to fishhealth@seafdec.org.ph. Alternatively, fax the deposit slip to (033) 3307011 c/o Fish Health Section.
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- Cash payment: Cash payment is made to the Cashier of SEAFDEC/AQD in Tigbauan, Iloilo by presenting a copy of the Statement of Account. Present the official receipt to the Fish Health Section.
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- Check payment: Checks should be made payable to the Southeast Asian Fisheries Development Center Aquaculture Department and mail to the Fish Health Section together with a copy of the Statement of Account.
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- The Fish Health Section will release the result, if ready, upon receipt of the proof of payment.
Ways of sending samples:
- Live samples
Appropriate for examinations such as:
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- Microscopy
- Bacteriology
- Mycology
- Virology (PCR and tissue culture)
- Histopathology
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- Iced samples
Appropriate for examinations such as:
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- Microscopy
- Bacteriology
- Mycology
- Virology
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- Fixed samples
Appropriate for examinations such as:
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- Virology (PCR)
- Histopathology
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Method of sampling for:
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Fish Health Laboratory Services Brochure (2023) Download PDF Format— File Size: 2.66 MB |
Watch related video!
Know more about the diagnostic services of SEAFDEC/AQD and how to send samples by watching this video: SEAFDEC/AQD Fish Health Diagnostic Services.
References
1 Standard Methods for the Examination of Water and Waste Waters. 2017. American Public Health Association. 23rd ed.
2 Bacteriological Analytical Manual. US FDA. (online) fda.food/gov/laboratory
3 Aflatoxin rapid test kit (Elabscience, 2018)
4 Humason, G. 1972. Animal Tissue Techniques. 3rd ed. WH Freeman and Company, USA p. 34-47.
5 Bell, T. A. and Lightner, D.V. 1998. A Handbook of normal penaeid shrimp histology. The World Aquaculture Society. Allen Press Inc., Lawrence, Kansas
6 IQ2000TM Detection and Prevention System, GeneReach Biotechnology Corp., Taiwan
7 Tang, KFJ and Lightner, DV. 2001. Detection and quantification of infectious hypodermal and hematopoietic necrosis virus in penaeid shrimp by real-time PCR. Diseases of Aquatic Organisms, 44(2):79-85.
8 Durand, SV and Lightner, DV. 2002. Quantitative real time PCR for the measurement of white spot syndrome virus in shrimp. Journal of Fish Diseases, 25: 381–389.
9 Dangtip S. et al, (2015). AP4 method for two-tube nested PCR detection of AHPND isolates of
Vibrio parahaemolyticus. Aquaculture Reports, 2:158-162.
10 Kurita J, Nakajima K, Hirono I and Aoki T. 1998. Polymerase chain reaction (PCR) amplification of DNA of red sea bream iridovirus (RSIV) Fish Pathol. 33(1):17-23.
11 Dong HT, Siriroob S, Meemetta W, Santimanawong W, Gangnonngiw W, Pirarat N, Khunrae P, Rattanarojpong T, Vanichviriyakit R and Senapin S. 2017. Emergence of tilapia lake virus in Thailand and an alternative semi-nested RT-PCR for detection. Aquaculture 476: 111-118.
12 Sri Widada J, Richard V, Shi Z, Qian D, Bonami JR. Dot-blot hybridization and RT-PCR detection of extra small virus (XSV) associated with white tail disease of prawn Macrobrachium rosenbergii. Dis Aquat Organ. 2004 Jan 28;58(1):83-7
13 Hameed ASS, Yoganandhan K, Widada JS and Bonami JR. 2004. Studies on the occurrence of Macrobrachium rosenbergii nodavirus and extra small virus-like particles associated with white tail disease of M. rosenbergii in India by RT-PCR detection. Aquaculture 238(1–4): pp127-133.